| MULTIGO |
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MultiGO is a server to detect statistically over-represented gene ontology (GO) terms
from hierarchically clustered gene expression data. Every hierarchically clustered
expression data set can be described as a gene tree. The tree shows the order in which the
genes are combined and their similarity, i.e. similarly expressed genes are combined
earlier than differently expressed. The created tree contains as many clusters as there are
genes in the experiment and in it genes can simultaneously belong to multiple nested
clusters, making the systematic analysis of gene functions of every cluster complicated and
impractical. The novel feature of MultiGO is that it is able to automatically analyzes each
cluster of the tree. It also the first web-enabled tool to this, according to our knowledge.
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In MultiGO hierarchically clustered gene expression data must be submited as GTR and
CDT-files. These files can be generated using the most common clustering tools. In
TIGR Mev after you have clustered your data, select the tree, right click your
mouse over the expression matrix and save Gene Node Heights (gtr-file) and
Save Gene Order (cdt-file) from the menu. In Cluster, Cluster 3.0
and in TreeView the cdt and gtr files are automatically generated. We are currently
not aware how to create cdt/gtr files in GeneSpring, but we are working with this.
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MultiGO was tested using Internet Explorer (6.0) in Windows environmet with no apparent
problems. Opera browsers are also compatible.
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